If it comes to speak about the future possibilities of molecular biology, it is worth keeping an eye on the medical applications. And if you think about the most peculiar infection agents you should for sure think of viruses also. What is a virus? Of course we all know what a virus might cause to us, [...]
Filed under: cell culture, DNA, molecular biology, RNA, virus | 2 Comments »
Restriction enzymes are used to cut plasmids. We have tackled the plasmids in the previous lecture. You can have a full description about the restriction enzymes here. As a most basic introduction I would say that restriction enzymes are enzymes of the bacteria representing a kind of immune function of the bacteria. They are present [...]
Filed under: DNA, molecular biology, plasmid, transfection, Water | Tagged: Lab, molecular biology, plasmid, Restriction Enzyme | Comments Off
Green lights in the dark When someone first shows up in our lab, the prime goal I set up for him or her is to make “green cells” - I mean to introduce a Green Fluorescent Protein into a mammalian cell culture. In order to be able to perform this one has to know some basic molecular [...]
Filed under: animation, cell, DNA, GFP, molecular biology, plasmid, RNA, transfection | Tagged: Central Dogma, DNA, DNA isolation, DNAses, EDTA, FRET, GFP, Green fluorescent protein, Jellyfish, RNA, Transcription, Translation | 4 Comments »
Dear Colleagues, I promised you to give an update about serological pipettes. We use serological pipettes when we want to manipulate (to move) liquids that are in the range of 5 to 25 ml. Smaller volumes than 5ml can be measured with Gilson type pipettes, while for larger volumes than 25ml we use measuring cylinders. [...]
Filed under: cell culture, Lab equipment, molecular biology, Pipet, sterile | Tagged: Pipettes, Serological Pipettes | 2 Comments »
