Designing QPCR oligos might seem complicated but there are some rules and softwares that can make it easy. In our lab, majority of data points we generate are probably measured by QPCR, so I think it is worth to review an algorithm for desiging QPCR assays. So, today I will describe the way I design QPCR oligos. You can have [...]
Filed under: DNA, PCR, Polymerase Chain Reaction | Tagged: fdc238433b8b23c811fed83d8cf09973, Oligo, oligodesign, PCR, QPCR, UCSC, UPL. LNA | Comments Off
