Transformation of competent cells

Scientific Background Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent. Some bacteria are naturally competent (e.g B. subtilis), whereas others such as E. coli are not naturally competent. Non-competent cells can be made competent and then transformed [...]

Green Fluorescent Protein or GFP

Green lights in the dark When someone first shows up in our lab, the prime goal I set up for him or her is to make “green cells” - I mean to introduce a Green Fluorescent Protein into a mammalian cell culture. In order to be able to perform this one has to know some basic molecular [...]

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