In this self-guided slideshare presentation you will learn the basics of High Resolution Melt Analysis HRM, applications, important considerations, assay via Tons of Resources for High Resolution Melt Analysis.
Filed under: molecular biology | Comments Off
Scientific Background Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent. Some bacteria are naturally competent (e.g B. subtilis), whereas others such as E. coli are not naturally competent. Non-competent cells can be made competent and then transformed [...]
Filed under: DNA, GFP, molecular biology, plasmid, Resources, Restriction Enzyme, transformation | Tagged: bacteria, Biology, DNA, GFP, Green fluorescent protein, Lab, plasmid, Restriction Enzyme | Comments Off
The Bill & Melinda Gates Foundation is now accepting grant proposals for Round 7 of Grand Challenges Explorations, an initiative to encourage innovative and unconventional global health solutions. Applicants can be at any experience level; in any discipline; and from any organization, including colleges and universities, government laboratories, research institutions, non-profit organizations and for profit [...]
Filed under: molecular biology | Tagged: health, Medical and Health, research, science, world | Comments Off
We think videos can help a lot for newcommers into the molecular biology laboratory. One of the goals of our team (iGEM Debrecen 2010) was to generate high quality videos of basic lab protocols. All the protocolls can be viewed here but you can have an intro into Cell passaging righ now if you watch [...]
Filed under: cell culture, iGEM, Lab equipment, molecular biology, Resources, sterile, video | Comments Off
Dear colleagues, An excellent tool is available to get a feeling about molecular sizes in Biology. The tool is available here and was developped by “Learn Genetics” program from the University of Utah. You can have a short movie on it below, but please check the original one from here!!!
Filed under: animation, cell, Cool stuff, DNA, molecular biology | Tagged: cell, DNA, Golgi, Life, Mitochondria, virus, Water | Comments Off
When I first heard about the Polymerase Chain Reaction my first association was with the atomic bomb chain reaction. You know probably from your studies: the labile Uranium if receives a neutron it transformed to a stable Uranium isotope while several new neutrons are released. If these newly release neutrons meet novel labile Uranium atoms [...]
Filed under: animation, DNA, molecular biology, PCR, plasmid, Polymerase, Polymerase Chain Reaction, Replication, Taq | Tagged: 454, chain reaction, critical mass, dNTP, Emulsion PCR, FLX, Geyser, Hot sprigns, Karry Mullis, lagging strand, leading strand, Noble Prize, Okazaki fragments, Polymerase, Polymerase Chain Reaction, Pyrosequencing, Replication, Steamboat, Taq, Thermophylus aquaticus, Yellowstone | 3 Comments »
If it comes to speak about the future possibilities of molecular biology, it is worth keeping an eye on the medical applications. And if you think about the most peculiar infection agents you should for sure think of viruses also. What is a virus? Of course we all know what a virus might cause to us, [...]
Filed under: cell culture, DNA, molecular biology, RNA, virus | 2 Comments »
Restriction enzymes are used to cut plasmids. We have tackled the plasmids in the previous lecture. You can have a full description about the restriction enzymes here. As a most basic introduction I would say that restriction enzymes are enzymes of the bacteria representing a kind of immune function of the bacteria. They are present [...]
Filed under: DNA, molecular biology, plasmid, transfection, Water | Tagged: Lab, molecular biology, plasmid, Restriction Enzyme | Comments Off
Green lights in the dark When someone first shows up in our lab, the prime goal I set up for him or her is to make “green cells” - I mean to introduce a Green Fluorescent Protein into a mammalian cell culture. In order to be able to perform this one has to know some basic molecular [...]
Filed under: animation, cell, DNA, GFP, molecular biology, plasmid, RNA, transfection | Tagged: Central Dogma, DNA, DNA isolation, DNAses, EDTA, FRET, GFP, Green fluorescent protein, Jellyfish, RNA, Transcription, Translation | 4 Comments »
Dear Colleagues, I promised you to give an update about serological pipettes. We use serological pipettes when we want to manipulate (to move) liquids that are in the range of 5 to 25 ml. Smaller volumes than 5ml can be measured with Gilson type pipettes, while for larger volumes than 25ml we use measuring cylinders. [...]
Filed under: cell culture, Lab equipment, molecular biology, Pipet, sterile | Tagged: Pipettes, Serological Pipettes | 2 Comments »
