Posted on April 12, 2009 by balintblaszlo
When I first heard about the Polymerase Chain Reaction my first association was with the atomic bomb chain reaction. You know probably from your studies: the labile Uranium if receives a neutron it transformed to a stable Uranium isotope while several new neutrons are released. If these newly release neutrons meet novel labile Uranium atoms [...]
Filed under: DNA, PCR, Polymerase, Polymerase Chain Reaction, Replication, Taq, animation, molecular biology, plasmid | Tagged: Taq, Polymerase Chain Reaction, Polymerase, Thermophylus aquaticus, Hot sprigns, Geyser, Karry Mullis, Noble Prize, dNTP, FLX, 454, Pyrosequencing, Emulsion PCR, chain reaction, critical mass, Okazaki fragments, Replication, leading strand, lagging strand, Yellowstone, Steamboat | 3 Comments »
Posted on April 6, 2009 by balintblaszlo
Dear Colleagues, Sometimes it is good to have links that cover a topic. This is why I have collected here a bunch of links that might be useful for you in your work. You can have a good description of the methods used in restriction enzyme analysis here: Methodbook.net If you would like to use restriction [...]
Filed under: DNA, Resources, Restriction Enzyme, plasmid | Tagged: Restriction Enzyme, Resources | 1 Comment »
Posted on March 15, 2009 by balintblaszlo
Restriction enzymes are used to cut plasmids. We have tackled the plasmids in the previous lecture. You can have a full description about the restriction enzymes here. As a most basic introduction I would say that restriction enzymes are enzymes of the bacteria representing a kind of immune function of the bacteria. They are present [...]
Filed under: DNA, Water, molecular biology, plasmid, transfection | Tagged: Restriction Enzyme, plasmid, molecular biology, Lab | Leave a Comment »
Posted on March 7, 2009 by balintblaszlo
Green lights in the dark When someone first shows up in our lab, the prime goal I set up for him or her is to make “green cells” - I mean to introduce a Green Fluorescent Protein into a mammalian cell culture. In order to be able to perform this one has to know some basic molecular [...]
Filed under: DNA, GFP, RNA, animation, cell, molecular biology, plasmid, transfection | Tagged: GFP, Green fluorescent protein, DNA, RNA, Transcription, Translation, Central Dogma, Jellyfish, DNAses, EDTA, DNA isolation, FRET | 4 Comments »
