Designing QPCR oligos might seem complicated but there are some rules and softwares that can make it easy. In our lab, majority of data points we generate are probably measured by QPCR, so I think it is worth to review an algorithm for desiging QPCR assays.
So, today I will describe the way I design QPCR oligos. You can have a [...]
Filed under: DNA, PCR, Polymerase Chain Reaction | Tagged: fdc238433b8b23c811fed83d8cf09973, Oligo, oligodesign, PCR, QPCR, UCSC, UPL. LNA | Leave a Comment »
When I first heard about the Polymerase Chain Reaction my first association was with the atomic bomb chain reaction. You know probably from your studies: the labile Uranium if receives a neutron it transformed to a stable Uranium isotope while several new neutrons are released. If these newly release neutrons meet novel labile Uranium atoms [...]
Filed under: DNA, PCR, Polymerase, Polymerase Chain Reaction, Replication, Taq, animation, molecular biology, plasmid | Tagged: 454, chain reaction, critical mass, dNTP, Emulsion PCR, FLX, Geyser, Hot sprigns, Karry Mullis, lagging strand, leading strand, Noble Prize, Okazaki fragments, Polymerase, Polymerase Chain Reaction, Pyrosequencing, Replication, Steamboat, Taq, Thermophylus aquaticus, Yellowstone | 3 Comments »
