Posted on June 8, 2010 by balintblaszlo
On this tutorial you can see an easy way to design oligos for gene synthesis, or in other words Gene design. The description is based on the Instructional Videos from the iGEM website! Gene design in less than 10 minutes! Good luck!!! Some usefull tools: 1. Gene design web page 2. Tools at DNA20 3. [...]
Filed under: DNA, Gene design, Polymerase Chain Reaction, Resources, Restriction Enzyme, animation, iGEM | Tagged: DNA, Gene design, gene synthesis, iGEM, Oligo, PCR, Restriction Enzyme | Leave a Comment »
Posted on May 19, 2009 by balintblaszlo
Designing QPCR oligos might seem complicated but there are some rules and softwares that can make it easy. In our lab, majority of data points we generate are probably measured by QPCR, so I think it is worth to review an algorithm for desiging QPCR assays. So, today I will describe the way I design QPCR oligos. You can have [...]
Filed under: DNA, PCR, Polymerase Chain Reaction | Tagged: fdc238433b8b23c811fed83d8cf09973, Oligo, oligodesign, PCR, QPCR, UCSC, UPL. LNA | Leave a Comment »
Posted on April 12, 2009 by balintblaszlo
When I first heard about the Polymerase Chain Reaction my first association was with the atomic bomb chain reaction. You know probably from your studies: the labile Uranium if receives a neutron it transformed to a stable Uranium isotope while several new neutrons are released. If these newly release neutrons meet novel labile Uranium atoms [...]
Filed under: DNA, PCR, Polymerase, Polymerase Chain Reaction, Replication, Taq, animation, molecular biology, plasmid | Tagged: Taq, Polymerase Chain Reaction, Polymerase, Thermophylus aquaticus, Hot sprigns, Geyser, Karry Mullis, Noble Prize, dNTP, FLX, 454, Pyrosequencing, Emulsion PCR, chain reaction, critical mass, Okazaki fragments, Replication, leading strand, lagging strand, Yellowstone, Steamboat | 3 Comments »
