Transformation of competent cells

Scientific Background Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent. Some bacteria are naturally competent (e.g B. subtilis), whereas others such as E. coli are not naturally competent. Non-competent cells can be made competent and then transformed [...]

Cell passaging video

We think videos can help a lot for newcommers into the molecular biology laboratory. One of the goals of our team (iGEM Debrecen 2010) was to generate high quality videos of basic lab protocols. All the protocolls can be viewed here but you can have an intro into Cell passaging righ now if you watch [...]

Why? This is the core question.

Why going in one particular direction if you can go in any direction? Why following someone, yes even in social media and by web2 tools? Only then comes the question “HOW” and at the very end “WHAT? Yes, the very last questions are WHAT TO DO. We have to know why we do something then [...]

Melinda Gates about Polio and Coca-Cola

Video project of the iGEM Debrecen Team

Beside the whole project as beeing done, we completed a very nice video project. See description here. The protocolls can be found here. The team wiki for the Debrecen iGEM team is here. Good luck!

Designing a gene or “Gene design”

On this tutorial you can see an easy way to design oligos for gene synthesis, or in other words Gene design. The description is based on the Instructional Videos from the iGEM website! Gene design in less than 10 minutes! Good luck!!! Some usefull tools: 1. Gene design web page 2. Tools at DNA20 3. [...]

Restriction Enzyme Resources

Dear Colleagues, Sometimes it is good to haveĀ links that cover a topic. This is why I have collected here a bunch of links that might be useful for you in your work. You can have a good description of the methods used in restriction enzyme analysis here: Methodbook.net If you would like to use restriction [...]

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