Transformation of competent cells

Scientific Background Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent. Some bacteria are naturally competent (e.g B. subtilis), whereas others such as E. coli are not naturally competent. Non-competent cells can be made competent and then transformed [...]

Designing a gene or “Gene design”

On this tutorial you can see an easy way to design oligos for gene synthesis, or in other words Gene design. The description is based on the Instructional Videos from the iGEM website! Gene design in less than 10 minutes! Good luck!!! Some usefull tools: 1. Gene design web page 2. Tools at DNA20 3. [...]

Restriction Enzyme Resources

Dear Colleagues, Sometimes it is good to haveĀ links that cover a topic. This is why I have collected here a bunch of links that might be useful for you in your work. You can have a good description of the methods used in restriction enzyme analysis here: Methodbook.net If you would like to use restriction [...]

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