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Archive for the ‘sterile’ Category

Cell passaging video

In cell culture, iGEM, Lab equipment, molecular biology, Resources, sterile, video on March 9, 2011 at 9:48 pm

We think videos can help a lot for newcommers into the molecular biology laboratory.

One of the goals of our team (iGEM Debrecen 2010) was to generate high quality videos of basic lab protocols. All the protocolls can be viewed here but you can have an intro into Cell passaging righ now if you watch this video:

Using Serological Pipettes

In cell culture, Lab equipment, molecular biology, Pipet, sterile on March 2, 2009 at 2:07 pm
Dear Colleagues,
I promised you to give an update about serological pipettes.
We use serological pipettes when we want to manipulate (to move) liquids that are in the range of 5 to 25 ml. Smaller volumes than 5ml can be measured with Gilson type pipettes, while for larger volumes than 25ml we use measuring cylinders.
Serological pipettes have a dispensable graduated tube, and a filter that is not allowing contamination with any particles from the air. The pipettes look like this for example:
serological-pipette

serological-pipette

They can be charged, have a button to move liquids up and one for release the aspirated liquids.

We use sterile, single packed pipettes in three different ranges: up to 5ml, to 10ml and to 25ml. They have different color codes:

sterile-serological-pipettes

sterile-serological-pipettes

The same from their back:

back-of-the-sterile-serological-pipets

Please have a look to my demo of how to use them:

Settings of a typical serological pipette can be seen below. You can adjust the power of the pump that enables you to move volumes as small as 1/10 of ml. And you can switch between “drop wise” and “blow out” mode. You use “drop wise” mode when you do not want to disturb the cells on the bottom of a culturing dish.

head-of-serological-pipette

head-of-serological-pipette

A detailed demo about serological and other similar type of pipettes:

What about using Gilson type pipettes in the cell culture lab? We have sterile, pyrogenic free tips for the cell culture lab. This box contains for example certified DNAse, RNase and Pyrogen free tips. Each tip has an individual filter insert! You can use them for probably any protocoll in a standard molecular biology lab. Don’t forget, they are not cheap at all…

barrier-tip-box-1ml

barrier-tip-box-1ml

barrier-tip-1ml

barrier-tip-1ml

Smaller volumes can be measured with smaller barrier tips:

barrier-tip-10ul

barrier-tip-10ul

I think we should go to the cell culture lab in one of our next post!

That’s all for today, and let me have your feedback!

Plenty of good basic info regarding laboratory work is described <a href="”>here.

Cheers,

Balint.

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