Restriction enzymes are used to cut plasmids. We have tackled the plasmids in the previous lecture. You can have a full description about the restriction enzymes here.
As a most basic introduction I would say that restriction enzymes are enzymes of the bacteria representing a kind of immune function of the bacteria. They are present in [...]
Filed under: DNA, Water, molecular biology, plasmid, transfection | Tagged: Lab, molecular biology, plasmid, Restriction Enzyme | Leave a Comment »
Green lights in the dark
When someone first shows up in our lab, the prime goal I set up for him or her is to make “green cells” - I mean to introduce a Green Fluorescent Protein into a mammalian cell culture. In order to be able to perform this one has to know [...]
Filed under: DNA, GFP, RNA, animation, cell, molecular biology, plasmid, transfection | Tagged: Central Dogma, DNA, DNA isolation, DNAses, EDTA, FRET, GFP, Green fluorescent protein, Jellyfish, RNA, Transcription, Translation | 4 Comments »
