Restriction enzymes are used to cut plasmids. We have tackled the plasmids in the previous lecture. You can have a full description about the restriction enzymes here. As a most basic introduction I would say that restriction enzymes are enzymes of the bacteria representing a kind of immune function of the bacteria. They are present [...]
Filed under: DNA, molecular biology, plasmid, transfection, Water | Tagged: Lab, molecular biology, plasmid, Restriction Enzyme | Comments Off
Green lights in the dark When someone first shows up in our lab, the prime goal I set up for him or her is to make “green cells” - I mean to introduce a Green Fluorescent Protein into a mammalian cell culture. In order to be able to perform this one has to know some basic molecular [...]
Filed under: animation, cell, DNA, GFP, molecular biology, plasmid, RNA, transfection | Tagged: Central Dogma, DNA, DNA isolation, DNAses, EDTA, FRET, GFP, Green fluorescent protein, Jellyfish, RNA, Transcription, Translation | 4 Comments »
