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Restriction Enzyme Resources

In DNA, plasmid, Resources, Restriction Enzyme, Uncategorized on April 6, 2009 at 8:46 pm

Dear Colleagues,

Sometimes it is good to have links that cover a topic. This is why I have collected here a bunch of links that might be useful for you in your work.

You can have a good description of the methods used in restriction enzyme analysis here: Methodbook.net

If you would like to use restriction enzymes for your work, you can find a list of links of the best known restriction enzyme providers below.

New England Biolabs

Promega

Roche Applied Science: Benchmate

Invitrogen

Fermentas

If you want to start your work with these enzymes, please consult the protocol provided with the enzyme or check it at the website of the manufacturer.

Be sure you know what an isoschizomer is, what star activity is, or how you can make double digestion (details here and here).

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  1. To see exactly how restriction enzymes interact at the molecular check out the many animated Jmol images in this tutorial:
    http://biology.kenyon.edu/BMB/Jmol2007/1DFM/

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